Identification and Quantification of Genomic Repeats and Sample Contamination...
Contigs assembled from 454 reads from bacterial genomes demonstrate a range of read depths, with a number of contigs having a depth that is far higher than can be expected. For reference genome...
View ArticleIncreasing Prevalence of Unique Mutation Patterns in H5N1 Avian Influenza...
Highly pathogenic avian influenza H5N1 virus (HPAIV) continues to be a candidate of a further influenza virus pandemic. Egypt is the country worst affected by human cases of HPAIV H5N1 infection in...
View ArticleComplete Genome Sequence of Rothia mucilaginosa DY-18: A Clinical Isolate...
Rothia mucilaginosa is an opportunistic pathogen in the human oral cavity and pharynx. We found that R. mucilaginosa DY-18, a clinical isolate from a persistent apical periodontitis lesion, had...
View ArticleMolecular Diversity of miR390-Guided Transacting siRNA Precursor Genes in...
Transacting siRNA loci (TAS3-like) of a particular plant species are usually represented by several gene families. PCR-based approach was used as a phylogenetic profiling tool to probe genomic DNA...
View ArticleKRAS Codons 12 and 13 Mutation Analysis: A Comparative Study between Direct...
KRAS somatic mutations are found in 30–40% of colorectal cancer (CRC). Seven mutations in codons 12 and 13 of KRAS (95% of the observed human mutations) preclude the efficacy of anti-EGFR therapy for...
View ArticleGene Expression in Leaves of Susceptible Glycine max during Infection with...
Soybean rust is caused by the obligate biotrophic fungus Phakopsora pachyrhizi, an exotic pathogen causing important yield losses in soybean production. We used an mRNA-Seq strategy to analyze the...
View ArticleMolecular Cloning, Sequencing, and Characterization of a Putative...
Vanda Mimi Palmer, a hybrid of Vanda Tan Chay Yan and Vanda tessellata (Roxb.) Hk.f. ex G. Don, is cultivated as a potted ornamental plant mainly for its fragrance rather than its look. Plant...
View ArticleCost-Effective Paramagnetic Bead Technique for Purification of Cycle...
The quality of sequencing results depends greatly upon the quality and purity of the template as well as the purity of the fluorescently labeled products generated by cycle sequencing. Numerous...
View ArticleIdentification of Prophages and Prophage Remnants within the Genome of...
Bacterial whole genome sequencing has delivered an abundance of prophage sequences as a by-product and the analysis of these sequences revealed ways in which phages have affected the genome of their...
View ArticleMolecular Cloning, Modeling, and Characterization of Type 2 Metallothionein...
Plantago ovata Forsk is a medicinally important plant. Metallothioneins are cysteine rich proteins involved in the detoxification of heavy metals. Molecular cloning and modeling of MT from P. ovata is...
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